tlr3 agonist poly  (InvivoGen)

Journal: Frontiers in Bioengineering and Biotechnology

Article Title: Innate immune pathway activated mesenchymal stromal cells improve function and histologic outcomes in a rodent osteoarthritis model

doi: 10.3389/fbioe.2025.1525969

Figure Lengend Snippet: RNA sequencing analysis comparing transcriptomes of joint tissues from mice treated with TLR3-MSC versus MSC. Volcano plot of transcriptome from n = 5 biological replicates of TLR3-MSC versus MSC treated mouse knee joints (A) . X-axis shows fold change and y-axis shows FDR adjusted p -value, with significantly upregulated genes shown as red dots and significantly downregulated genes shows as blue dots. Significance defined as FDR ≤0.05 fold change ≥2 or ≤−2. Differential gene expression (list of genes, description, unadjusted p -value and fold-change of top 20) for upregulated (B) and downregulated (C) genes in differential analysis results from TLR3-MSC versus MSC treated joints. Pathway analyses of differential gene expression were performed using normalized counts from n = 5 biological replicates of mice treated with either TLR3-MSC or MSC alone, indicating the top upregulated (D) and downregulated (E) pathways. FC, Fold Change; FDR, false discovery rate; MSC, mesenchymal stromal cells; TLR, Toll-Like receptor; STING, Stimulator of Interferon Genes.

Article Snippet: At weeks 3 and 5, MSC were trypsinized, counted, and a portion activated with the TLR3 agonist polyinosinic-polycytidylic acid (pIC) (InVivoGen, San Diego, CA) or 2′3′cGAMP (InVivoGen, San Diego, CA) to stimulate cGAS-STING pathways (stimulation dose at 10 μg/mL at a concentration of 1 × 10 6 cells/mL in growth media for 2 h stimulation time).

Techniques: RNA Sequencing, Gene Expression

Journal: Frontiers in Bioengineering and Biotechnology

Article Title: Innate immune pathway activated mesenchymal stromal cells improve function and histologic outcomes in a rodent osteoarthritis model

doi: 10.3389/fbioe.2025.1525969

Figure Lengend Snippet: RNA sequencing analysis comparing transcriptomes of joint tissues from mice treated with STING-MSC versus TLR3-MSC. Volcano plot of transcriptome from n = 5 biological replicates of TLR3-MSC versus MSC treated mouse knee joints (A) . X-axis shows fold change and y-axis shows FDR adjusted p -value, with significantly upregulated genes shown as red dots and significantly downregulated genes shows as blue dots. Significance defined as FDR ≤0.05 fold change ≥2 or ≤−2. Venn diagram of differentially expressed genes for each comparison group and to resting MSC (B) . Differential gene expression (list of genes, description, unadjusted p -value and fold-change of top 20) for upregulated (C) and downregulated (D) genes in differential analysis results from TLR3-MSC versus MSC treated joints. Pathway analyses of differential gene expression were performed using normalized counts from n = 5 biological replicates of mice treated with either TLR3-MSC or MSC alone, indicating the top upregulated (E) and downregulated (F) pathways. FC, Fold Change; FDR, false discovery rate; MSC, mesenchymal stromal cells; TLR, Toll-Like receptor; STING, Stimulator of Interferon Genes.

Article Snippet: At weeks 3 and 5, MSC were trypsinized, counted, and a portion activated with the TLR3 agonist polyinosinic-polycytidylic acid (pIC) (InVivoGen, San Diego, CA) or 2′3′cGAMP (InVivoGen, San Diego, CA) to stimulate cGAS-STING pathways (stimulation dose at 10 μg/mL at a concentration of 1 × 10 6 cells/mL in growth media for 2 h stimulation time).

Techniques: RNA Sequencing, Comparison, Gene Expression

Journal: Frontiers in Bioengineering and Biotechnology

Article Title: Innate immune pathway activated mesenchymal stromal cells improve function and histologic outcomes in a rodent osteoarthritis model

doi: 10.3389/fbioe.2025.1525969

Figure Lengend Snippet: Histologic representative images of mouse knees at endterm. Toluidine blue photomicrographs from (left to right) control (needle insertion alone), MSC, TLR3-MSC, and STING-MSC treated (right) limbs. Low magnification (×4) images of whole knee joints from (left to right) control (A) , MSC (D) , TLR3-MSC (G) , and STING-MSC (J) treated joints. Higher magnification (×10) images presented for evaluation of the medial compartment of (left to right) control (B) , MSC (E) , TLR3-MSC (H) , and STING-MSC (K) treated joints. Additional higher magnification image for evaluation of the lateral compartment for control (C) , MSC (F) , TLR3-MSC (I) and STING-MSC (L) treated joints. (A,D,G,J) 4x, scale bar = 200 μm; (B,C,E,F,H,I,K,L) 10x, scale bar = 20 μm. Pink arrows represent mostly cartilagenous osteophytes. The yellow circle indicates synovitis. Red arrows highlight areas of cartilage loss to below the calcified layer. White arrows outline a roughened cartilage surface, as well as fibrillation. MSC, mesenchymal stromal cells; TLR, Toll-Like receptor; STING, Stimulator of Interferon Genes.

Article Snippet: At weeks 3 and 5, MSC were trypsinized, counted, and a portion activated with the TLR3 agonist polyinosinic-polycytidylic acid (pIC) (InVivoGen, San Diego, CA) or 2′3′cGAMP (InVivoGen, San Diego, CA) to stimulate cGAS-STING pathways (stimulation dose at 10 μg/mL at a concentration of 1 × 10 6 cells/mL in growth media for 2 h stimulation time).

Techniques: Control

Journal: Frontiers in Bioengineering and Biotechnology

Article Title: Innate immune pathway activated mesenchymal stromal cells improve function and histologic outcomes in a rodent osteoarthritis model

doi: 10.3389/fbioe.2025.1525969

Figure Lengend Snippet: OARSI histopathology scores. Scoring of histology slides was performed following DMM surgery and treatment with control (needle insertion alone), MSC, TLR3-MSC, or STING-MSC. Parameters listed include whole joint OARSI total score (A) , medial compartment total score (B) , lateral compartment total score (C) , medial tibial cartilage score (D) , medial femoral cartilage (E) , medial synovium score (F) , lateral tibial cartilage score (G) , lateral femoral cartilage score (H) , lateral synovium score (I) . Significant differences noted with p values < 0.05. MSC, mesenchymal stromal cells; TLR, Toll-Like receptor; STING, Stimulator of Interferon Genes.

Article Snippet: At weeks 3 and 5, MSC were trypsinized, counted, and a portion activated with the TLR3 agonist polyinosinic-polycytidylic acid (pIC) (InVivoGen, San Diego, CA) or 2′3′cGAMP (InVivoGen, San Diego, CA) to stimulate cGAS-STING pathways (stimulation dose at 10 μg/mL at a concentration of 1 × 10 6 cells/mL in growth media for 2 h stimulation time).

Techniques: Histopathology, Control